Introduction
The yT&A cloning kit contain the yT&A
vector and many reagents needed for
ligation. It is a convenient pack
for cloning PCR product generated
using thermostable DNA polymerases,
such as YEAtaq polymerase, which add
a single terminal 3’-dA nucleotide
overhang. After ligation, the
mixture can be used directly to
transform into competent cells (ECOSTM
perform better) or be purified first
to achieve higher transformation
efficiency. The kit contains enough
reagents for 20 reactions.
Benefits
Fast!! Ligation can be completed
within 5 ~ 20 minutes vs. 1 hour to overnight by using similar
systems from other companies.
Higher transformation efficiency in
comparison to other competitor’s cloning system.
More accurate results in comparison
to other companies in colony pickings.
Two types of ligation buffer
provided for your convenience.
Applications
Accepts terminal 3’-dA nucleotide
overhang PCR products
Transform ligation product
(purified/unpurified) into ECOSTM
or competent cells
Lac Z
complementation for blue/white
screening
Ampicillin marker for antibiotic
selection
Storage conditions
Store at –20°C
Cloning vector
(download cloning vector sequence)
With ampicillin resistance
With blue/white screening capability
All pass rigorous QC test to ensure
the background colonies are close to zero
Very stable
Before the insert incorporated into
the yT&A cloning vector, there is
only 1
HindIII
site and no
BglII
site. After the incorporation, the T
and A nucleotides on the insert
complement the sequence on the
vector and generate these two new
sites. This merit of yT&A vector
makes cloning more economic and
convenient.
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